Fully denaturing two-dimensional electrophoresis of membrane proteins: A critical update
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چکیده
منابع مشابه
Fully denaturing two-dimensional electrophoresis of membrane proteins: a critical update.
The quality and ease of proteomics analysis depends on the performance of the analytical tools used, and thus of the performances of the protein separation tools used to deconvolute complex protein samples. Among protein samples, membrane proteins are one of the most difficult sample classes, because of their hydrophobicity and embedment in the lipid bilayers. This review deals with the recent ...
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One third of all genes of various organisms encode membrane proteins, emphasizing their crucial cellular role. However, due to their high hydrophobicity, membrane proteins demonstrate low solubility and a high tendency for aggregation. Indeed, conventional two-dimensional gel electrophoresis (2-DE), a powerful electrophoretic method for the separation of complex protein samples that applies iso...
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the citrate medium described by Halestrap (1976) was used, since this generated a pH gradient (alkaline inside) which aided pyruvate and lactate accumulation. In other experiments buffered saline was used as described by Spencer & Lehninger (1976) in order to resemble the physiological situation more closely. Details of the conditions are given in the legends to the Figure and Table. In Fig. 1 ...
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ژورنال
عنوان ژورنال: PROTEOMICS
سال: 2008
ISSN: 1615-9853,1615-9861
DOI: 10.1002/pmic.200800043